1.) Click on the photo below. A new Browser Window should pop-up resembling the photograph.

2.) Search "Nucleotide" for "AF008216" by typing it into the search box (highlighted in red above).

3.) Click on Go.

4.) Click on the hyperlink "AF008216"

5.) Print a copy of the document! (The entire document is 3 pages in length).

Questions

1. Identify the source organism and the chromosome from which the sequence has been obtained.

2. Identify the codons, using the base sequence numbers, from which the tumor suppressor protein
   is translated.  For example, bps 9011-9016 (OBVIOUSLY, THIS IS NOT AN ANSWER)

3. Identify the Eco RI and Hha I cut site(s) on the gene's base sequence using different colors.
   ***Remember, there is a listing of restriction enzymes off of the main page.***

4. How many fragments is this gene divided into after reaction with the previously mentioned
   restriction enzymes?

5. Describe in your own words, how the restriction enzyme fragments produced, could be inserted into
   an E.coli plasmid.

Last Revised: 6/21/2001 12:37
TJ Adase